Determination of ephedrine alkaloids in Ephedra sinica by nonaqueous capillary electrophoresis
- VernacularTitle:麻黄中生物碱类有效成分的非水毛细管电泳含量测定
- Author:
Yibing JI
;
Yuying CHEN
;
Rujin WU
;
- Publication Type:Journal Article
- Keywords:
Ephedra sinica Stapf;
ephedrine alkaloids;
nonaqueous capillary electrophoresis (NACE);
quantitative analysis
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a rapid method for the determination of ephedrine alkaloids in Ephedra sinica Stapf by nonaqueous capillary electrophoresis (NACE) and evaluate the extracting method by determining the amount of alkaloids Methods The buffer contained 50 mmol/L ammonium acetate in methanol without any additives was used And the detection wavelength was 210 nm Results The best separation result was achieved within 8 min Linearity was obtained in range of 9 8-147 0 ?g/mL pseudoephedrine, 6 8-102 0 ?g/mL for norephedrin, 9 4-141 0 ?g/mL for ephedrine, 4 8-72 0 ?g/mL for norpseudoephdrine, 6 8-102 0 ?g/mL for methylephedrine respectively The recovery range of these five alkaloids was 95 0%-100 4% Conclusion This method is rapid and accurate for the quantitative analysis of ephedrin alkaloids in E sinica
