STUDIES ON THE CLONAL GROWTH AND PASSAGE OF HUMAN EMBRYONIC STEM CELLS
- VernacularTitle:人胚胎干细胞原代克隆生长及其传代的研究
- Author:
Tao LI
;
Qingyun MAI
;
Canquan ZHOU
;
Guanglun ZHUANG
;
- Publication Type:Journal Article
- Keywords:
Human embryonic stem cells;
Passage;
Cell line;
Blastocyst
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Try to determine the relationship between blastocyst quality,the clonal growth of inner cell mass(ICM)and the establishment of human embryonic cell line. Methods Coculture D 3 discarded embryos with mouse embryonic fibroblast cells(MEFs).Then remove trophoectoderm by immunosurgery after getting different quality blastocysts.Culture ICM and passage these cells on MEFs. Results Human embryonic stem cells derived from good quality blastocysts could be passaged further than that from poor quality blastocysts,and ICMs growing fast could be passaged more quickly and efficiently.Conclusion The establishment of human embryonic stem cells is closely related with blastocyst quality and the original growth of ICM.
