Effects of recombined rat transforming growth factor beta 1 and insulin-like growth factor 1 transfected rabbit chondrocytes on their proliferation and synthesis ex vivo
- VernacularTitle:重组大鼠转化生长因子-?_1和胰岛素样生长因子-1基因转染兔软骨细胞及对其增生及合成的促进作用
- Author:
Chuan XIANG
;
Jingyuan DU
;
Xiaochun WEI
;
- Publication Type:Journal Article
- Keywords:
Chondrocytes;
Transforming growth factor beta;
Gene therapy;
Transfection
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether rabbit articular chondrocytes express growth factor genes delivered by recombined rat TGF beta 1,IGF 1 and what other influences on chondrocytes are,and to determine which gene is the best one for osteoarthritis therapy.Methods Monolayer cultures of rabbit articular chondrocytes were infected with recombinant plasmid pcDNA 3 and pAT 153 carrying genes encoding the following growth factors respectively:TGF beta 1 and IGF 1.The synthesis of TGF beta 1,IGF 1 and type Ⅱ collagen was measured by in situ hybridization,immunohistochemistry,immunofluoroscopy,flow cytometer and 3 H TdR radiolabeling.Results The expression of TGF beta1,IGF 1 and type Ⅱ collagen was high beyond control levels ( P
