Determination of catechins and caffeine in tea polyphenols
- VernacularTitle:茶多酚中儿茶素类及咖啡因的含量测定
- Author:
Yu HE
;
Xiaokun HONG
;
Zhihua WANG
;
- Publication Type:Journal Article
- Keywords:
tea polyphenols;
catechins;
caffeine;
HPLC;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a HPLC method for simultaneous determination of catechins (EGC、DC、EGCG、EC、GCG、ECG) and caffeine in tea polyphenols and to quantify seven ingredients in twenty nine batches of tea polyphenols samples from thirteen producers. Methods: The study was achieved using a C 18 column with a methanol water 0.1% formic acid gradient elution system. Results: The peak resolutions of seven ingredients in mixed standards were all above 2; The resolutions in samples were all above 1.5 except EGCG; The average recoveries were 98.56%～100.14%. Conclusion: This method is accurate, stable, reproducible and suitable for quality control of tea polyphenols; the variations of contents are obvious among tea polyphenols samples from different even the same producers.
