Biotransformation of cantharidin by cell suspension cultures of Platycodon grandiflorus
- VernacularTitle:桔梗细胞悬浮培养体系对斑蝥素的生物转化研究
- Author:
Min YE
;
Jungui DAI
;
Dean GUO
;
- Publication Type:Journal Article
- Keywords:
cantharidin;
Platycodon grandiflorus (Jacq ) A DC;
cell suspension culture;
biotransformation
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To investigate the biotransformation of cantharidin by cell suspension cultures of Platycodon grandiflorus (Jacq ) A DC Methods Cantharidin was added into cell suspension cultures of P grandiflorus and incubated for another six days The culture supernatant was extracted with EtOAc and then subjected to silica gel chromatography Results Two products were isolated as a mixture in a molar ratio of about 2∶1 Their structures were identified on the basis of spectroscopic data as 1? OH cantharidin (Ⅱa) and 1? OH cantharidin (Ⅱb) The relative configuration was elucidated according to NOESY spectrum Conclusion The two products were new cantharidin derivatives
