Determination of lignans of Schisandra rubriflora by HPLC
- VernacularTitle:HPLC法测定红花五味子木脂素的含量
- Author:
Yanhan WANG
;
Jianping GAO
;
Yunqiu YU
;
Daofeng CHEN
;
- Publication Type:Journal Article
- Keywords:
Schisandra rubriflora (Franch ) Rehd et Wils;
lignans;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils , which were collected from different areas Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm?4 6 mm, 5 ?m); mobil phase, A: H 2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora
