Effect of growth regulators on rooting of regenerated shoots from Dendrobium huoshanense protocorm-like bodies
- VernacularTitle:生长调节物质对霍山石斛试管苗生根的影响
- Author:
Wei SHI
;
Jianping LUO
;
Xiuyan HUANG
;
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense C Z Tang et S J Cheng;
regenerated shoot;
rooting;
growth regulators
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the effects of different growth regulators and organic additives on rooting of regenerated shoots from Dendrobium huoshanense C Z Tang et S J Cheng protocorm like bodies Methods In order to induce root development, the regenerated shoots were cultured on MS medium containing 3% sucrose and 0 8% agar and supplemented with NAA, IBA, BA, KT, banana mud or amino acids at different concentrations During rooting induction, the development of roots was observed and the frequency of rooted shoots was scored Results The frequency and the process of root initiation were significantly influenced by different growth regulators or organic additives For initiation and development of roots, the suitable concentration of NAA, IBA, BA, KT, banana mud or amino acids was 0 2 , 0 1, 0 8, 0 8 mg/L, 20% and 0 1%, respectively Conclusion In all media tested, NAA, IBA or banana mud display greater effects on rooting of regenerated shoots of D huoshanense than others and KT enables shoots to keep green
