Comparison of three methods for the determination of cholic acids in Qingkailing Injection
- VernacularTitle:清开灵注射液中三种胆酸含量测定方法比较
- Author:
Jin CAO
;
Yan XU
;
Yiming WANG
;
Guoan LUO
;
- Publication Type:Journal Article
- Keywords:
Qingkailing Injection, cholic acid, ELSD
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish two methods (HPLC UV and HPLC ELSD)for the determination of 3 kinds of cholic acids in Qingkailing Injection, and compare them with UV method. Methods: Two HPLC methods detected by UV and ELSD, respectively have been developed: Phenomenex Luna C 18 column(250mm?4.6mm,5?)with linear gradient elution using methanol acetotrile(4∶1) and 0.1% aqueous formic acid. Results: Three kinds of cholic acids in Qingkailing could be determined simultaneously by HPLC UV and HPLC ELSD. The recovery of three method were 100%,97%,95%～96% for HPLC/ELSD,HPLC/UV,UV, respectively. Conclusion: The HPLC ELSD method was accurate and specific to determine the content of cholic acids in Qingkailing Injection.
