Experimental study of establishing mouse model for scleroderma
- VernacularTitle:建立硬皮病小鼠模型的实验研究
- Author:
Wenzhen TU
;
Kai ZHAO
;
Lei LU
;
- Publication Type:Journal Article
- Keywords:
Mice,inbred C3H;
Mice,inbred BALB C;
Scleroderma,circumscribed;
Bleomycin
- From:
Chinese Journal of Rheumatology
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model for scleroderma.Methods Forteen BALB C and 14 C3H female mice were averagely divided into model 1 and controls.Daily 0 1 ml BLM at a concentration of 200 ?g/ml was injected intracutaneusly into the backs of model 1 mice for 3 weeks,and 0 1 ml solution of PBS were injected intracutaneusly into the backs of control mice for 3 weeks.Observing the histological change of skin and lungs was made and measuring the thickness of dermis was performed with a medical analysis system of the color picture,determined the collagenic quantity was done with photoelectric colorimetry,and calculating the immunohistochemical index of collagen type Ⅰ and Ⅲ and transforming growth factor ? 1 (TGF ? 1) in the skin lesions from the mouse model and control was done.SPSS was used to finish the statistical analysis of the detective value from model 1 and controls.Results In the skin of model mice,the thickness of dermis markedly thickened ( P
