THE RELATIONSHIPS BETWEEN PARAMETERS OF CELL KINETICS AND EXPRESSION, MUTATION OF RELATED GENES IN BREAST CANCER
- VernacularTitle:乳腺癌细胞动力学指标与相关基因表达、突变的关系
- Author:
Hongzhi LI
;
Kang YU
;
Zongmin WANG
;
Liwei XIE
;
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Flow cytometry;
Immunohistochemistry;
PCR SSCP.
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationships between cell kinetics, apoptosis and expression, mutation of related genes in breast cancer. Methods Flow cytometry, immunohistochemical SP and PCR SSCP were used respectively to study DNA index(DI), S phase fraction(SPF), proliferation index(PI), apoptosis index(AI), expression of c erbB 2, Bcl 2, p53, PCNA, Ki67, TopoⅡ and mutation of p53 in 54 cases of breast cancer. Results High DI, aneuploidy rate, SPF, PI and AI were coincided with high expression of c erbB 2, p53, PCNA, Ki167 and TopoⅡ. Low DI, SPF, PI and AI were coincided with high expression of Bcl 2. High DI, SPF, PI and AI were coincided with high mutation of p53. High AI was related to a type of p53 mutation——loss of heterozygosity(LOH). Conclusion Abnormal cell kinetics and apoptosis were related with abnormal expression, mutation of related genes in breast cancer. [
