Polymerase chain reaction analysis for the tumor necrosis factor alpha-308 (G>A) gene polymorphism in relation to ankylosing spondylitis
- VernacularTitle:肿瘤坏死因子-?-308基因多态性与强直性脊柱炎相关性研究
- Author:
Jingan LIN
;
Defu YE
;
Junmin CHEN
;
Weiqing ZHENG
;
Yueyong ZHU
;
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor;
Genotype;
Spondylitis,ankylosing;
Polymerase chain reaction
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between tumor necrosis factor ? (TNF ?) gene polymorphism and ankylosing spondylitis (AS).Methods Genomic DNA from 98 Chinese AS patients and 70 ethnically matched controls were typed for TNF(308) polymorphism by allele specific polymerase chain reaction (AS PCR).Results The TNF genotypes in AS patients were respectively TNF1 homozygote 37%,TNF2 homozygote 10% and TNF1 and TNF2 heterozygote 53%.While TNF genotypes in controls group were respectively TNF1 homozygote 67%,TNF2 homozygote 3% and TNF1 and TNF2 heterozygote 30%.Significant difference was found in the distribution of TNF 308 genotype between both groups ( ? 2=15 73, P
