Study on quality control of melittin by SDS-PAGE method
- VernacularTitle:蜂毒素的SDS-PAGE质控方法研究
- Author:
Dongxia ZHAI
;
Ling LIU
;
Yani ZHANG
;
Changquan LING
;
- Publication Type:Journal Article
- Keywords:
melittin;
SDS PAGE;
silver dyed;
gel image scanning
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish a SDS PAGE method on quality control of melittin. Methods Melittin was extracted and purified from bee venom by gel chromatography. Purity and molecular mass of melittin were determined by SDS PAGE. Results In the gelatinous board, the sample was a single peptide chain with a molecular mass of about 2 800 dalton as revealed by SDS PAGE. The purity was (90 59?0 85)%, which was in parallel with the result tested by HPLC. Conclusion SDS PAGE is accurate, reliable and simple for the determination of melittin.
