Production of puerarin and other isoflavones in cell suspension cultures in leaves of Pueraria lobata seedling
- VernacularTitle:野葛幼叶细胞悬浮培养生产葛根素等异黄酮类化合物的研究
- Author:
Chunrong ZHANG
;
Ling LI
;
- Publication Type:Journal Article
- Keywords:
Pueraria lobata (Willd.) Ohwi;
suspension culture;
puerarin;
isoflavones
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To establish cell suspension culture system in leaves of seedling of Pueraria lobata (Willd.) Ohwi Methods The callus induced from leaf explants of P.lobata seedling were suspended in liquid medium. The time courses of cell growth and yields of puerarin and isoflavones were studied Results The log phase of the cell cultures' growth was from 1st d through 5th d. The dry weight accumulation reached highest on 8th d. The production of puerarin was highly coupled with the cell growth. The yield of puerarin reached highest on 8th d. However, the accumulation of total isoflavones was different from that of puerarin. The yield of total isoflaones reached the highest on 10th d Conclusion The yield of total isoflavones in cell suspension culture system is higher than that in wild plants, while the yield of puerarin is lower than that in wild plants
