Expression of telomerase in human ovarian luteinized granulosa cells and its relationship to ovarian function
- VernacularTitle:黄素化颗粒细胞端粒酶的表达及与卵巢功能关系的初步研究
- Author:
Wen LIU
;
Guijin ZHU
;
- Publication Type:Journal Article
- Keywords:
Telomerase;
Granulosa cells;
Ovary
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of telomerase catalytic subunit (TERT),telomerase in human ovarian luteinized granulosa cells and to investigate the relationship between telomerase expression and the ovarian fecundity Methods Ovarian luteinized granulosa cells were recovered from 22 women with regular menses who underwent in vitro fertilization/ intracytoplasmic sperm injection programme.We carried out in situ hybridization histochemistry on luteinized granulosa cells to detect TERT mRNA expression,and telomeric repeat amplification protocol to detect telomerase activity Results TERT mRNA were present in luteinized granulosa cells.The expression of TERT mRNA and telomerase activity in ovarian luteinized granulosa cells decreased with increase of age,basal serum follicle stimulating hormone levels Conclusions These results suggest that telomerase may play an active role in ovarian function.A reduced telomerase activity of granulosa cells may be one of the important mechanisms involved in decreased ovarian function in women.
