Content Determination of Brucine and Strychnine in Tongbiling Tablet by Reversed-phase HPLC
- VernacularTitle:反相高效液相法测定通痹灵片中马钱子碱、士的宁的含量
- Author:
Ke'Er HUANG
;
Xuehong KE
;
Jifan CHEN
;
- Publication Type:Journal Article
- Keywords:
TONGBILING/analysis;
BRUCINE/chemistiy;
STRYCHNINE/chemistry;
GHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a method for the determination of brucine and strychnine contents in Tongbiling Tablet. [Methods] Reversed-phase HPLC conditions were: Nucleodur C18 Gravity column (125 mm ? 4.6 mm, 5?m), mobile phrase being methanol-water (60:40) with 0.5 g potassium dihydrogen phosphate and 1.0 g dodecyl sodium sulfouate and 0.5 mL triethylamine in 1 000 mL mixture (adjusting pH to 4.5 with acetic acid) , detecting wavelength at 254 run and a flow rate of 1.0 mL/min at room temperature. [ Results ] The average recovery of brucine was 97.69 % ( RSD = 2.03%) and that of strychnine 97.93% ( RSD = 2.61%). [Conclusion] This method is efficient and can be used for the quality control of Tongbiling Tablet.
