Inhibition effects of c-erbB-2 and c-raf-1 antisense oligodeoxynucleotides combined transfection on the human ovarian carcinoma transplanted subcutaneously in nude mice
- VernacularTitle:c-erbB-2与c-raf-1反义寡核苷酸联合转染对裸鼠人卵巢癌皮下移植瘤形成的抑制作用
- Author:
Yongzhong WU
;
Qinglan REN
;
Sufen YANG
;
Xiaopin CHEN
;
Shaolin LI
;
- Publication Type:Journal Article
- Keywords:
Oligonucleotides,antisense;
Transfection;
Ovarian neoplasms;
Carcinoma;
Neoplasm transplantation
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition effects of c erbB 2 and c raf 1 antisense oligodeoxynucleotides (ASODN) combined transfection on the human ovarian epithelial cancer transplanted subcutaneously in nude mice Methods There were 7 groups: normal control group,c erbB 2 sense observed group,c raf 1 sense observed group,c erbB 2 antisense observed group,c raf 1 antisense observed group,whole dose combined group,half dose combined group Human ovarian epithelial cancer cells SKOV3 were treated by different oligodeoxynucleotides,then transplanted subcutaneously in nude mice,respectively The changes of tumor volume were observed and the tumor growth inhibitory rate was calculated Results There was no difference between sense observed group and normal control group There was a larger growth inhibitory rate in whole dose combined group and half dose combined group,the first time that can be detected was 13 7 days and 15 2 days,and the maximum tumor growth inhibitory rates were 61 1% and 71 3%,respectively Conclusions The results suggested that ASODN combined transfection can inhibit the tumorigenesis of ovarian epithelial cancer cells in nude mice,it may be a more useful gene therapy for the ovarian epithelial carcinoma
