ISOLATION AND CULTURE OF NEURAL STEM CELLS FROM BRAIN OF RAT
- VernacularTitle:大鼠脑源性神经干细胞的分离培养
- Author:
Hui YAN
;
Shumin JIN
;
Shifu ZHANG
;
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Growth factor;
Induced differentiation
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of optimized conditions on proliferation and differentiation of the neural stem cells(NSCs) from different age brain of rat in vitro. Methods The brain tissues from embryonic(E12～E15d),newborn(1～2?d) hippocampus and olfactory bulb from adult of rat were isolated.After proliferation in conditional culture medium including growth factor and induced differentiation,the cell type specific antibodies were used for the immune fluorescence staining to identify the cells. Results A lager scale NSCs were obtained from brain tissue and could proliferate in conditional culture in vitro. They could self renew and generate both nerve and glia.Conclusion\ The NSCs got from rat brain can be used for basic and clinical research.\;[
