STUDIES ON THE RELATIONS OF EXPRESSION OF ?1,4- GALACTOSYLTRANSFERASE-Ⅰ AND PROLIFERATION OF SCHWANN CELLS
- VernacularTitle:?1,4-半乳糖基转移酶-Ⅰ对施万细胞增殖的影响
- Author:
Aiguo SHEN
;
Fei DING
;
Xiaosong GU
;
Liang QIANG
;
Han WANG
;
Jianxin GU
;
- Publication Type:Journal Article
- Keywords:
GalactosyltransferaseⅠ;
Schwann cells;
Cell proliferation;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to study the effect of ? 1,4 GalT Ⅰ on proliferation of Schwann cells, The changes in cell proliferation were checked after Schwann cells transfected with sense or antisense ? 1,4 GalT Ⅰ plasmids. Methods Methods of counting numbers of proliferating cell and measuring [ 3H] thymidine incorporation by liquid scintillation were used to study the proliferation of purified Schwann cells which transfected with ? 1,4 GalT Ⅰ plasmids. Cell cycles of those transfected cells were determined by fluorescence activated cell sorting. Results After Schwann cells transfected with sense ? 1,4 GalT Ⅰ plasmids, the proliferation of those cells was restrained, and the number at G 1/G 0 phase of those cells increased while the number at S phase decreased. Schwann cells transfected with antisense ? 1,4 GalT Ⅰ plasmid showed opposite changes.Conclusion ? 1,4 GalT Ⅰ may play an important role in regulating the proliferation of Schwann cells in peripheral nerve.\;[
