TCR/CD3 complex-mediated signal transduction in T cells from patients with systemic lupus erythematosus
- VernacularTitle:系统性红斑狼疮患者T细胞TCR/CD3复合物介导的信号传导研究
- Author:
Qingyong YANG
;
Yuanxing LIAO
;
Zongfa WANG
;
Huilan YANG
;
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Calcium;
T Lymphocytes;
Receptor CD3 complex,antigen,T cell;
Signal transduction
- From:
Chinese Journal of Rheumatology
2002;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether systemic lupus erythematosus (SLE) T cell function disorder is related to abnormal biochemical pathways.Methods After cross linking of anti CD3 mAbs to sheep anti mouse IgG and stimulating T cells,the changes of free calcium ion within T cells and these changes under interference of Thapsigargin and EGTA were observed respectively for 10 minutes with an adhesion cytometry.The relation between [Ca 2+ ]i response in SLE T cells and expression of CD3 molecules,or InsP 3 levels was evaluated.Results The base [Ca 2+ ]i response in T cells of SLE patients was similar to that of normal control ( P =0 105).Peak and plateau [Ca 2+ ]i responses were significantly higher in the group of SLE patients ( P
