Research on the pharmacokinetic parameters of 6,7-dimethoxycoumarin after oral administration of Yinchenhao and its decoction
- VernacularTitle:茵陈蒿及其汤剂药物动力学比较研究
- Author:
Qian WANG
;
Zhiguo YU
;
Song XUE
;
- Publication Type:Journal Article
- Keywords:
Yinchenhao decoction;
6,7 dimethoxycoumarin;
pharmacokinetics;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the plasma concentrations and pharmacokinetic parameters of 6,7 dimethoxycoumarin after oral administration of Herba Artemisiae Scopariae decoction and Yinchenhao decoction. Methods: A HPLC UV method was developed to determine 6,7 dimethoxycoumarin in rat plasma. The drug was extracted with acetonitrile from plasma and separated on a Kromasil ODS (250mm?4.6mm,5?m)column with methanol 1% solution of acetic acid THF (30∶63∶7) as mobile phase, UV detecter was set at 340nm. The data obtained were analyzed with Topfit program. Results: The liner calibration curves were obtained in the concentration range of 0.026～2.5 ?g?mL -1 . With non compartmental analysis, the main pharmacokinetic parameters after oral administration of Artemisiae Scopariae decoction and Yinchenhao decoction were as follows: T 1/2 was 1.30 and 1.75h, AUC 0→t was 1215.00 and 2527.85ng?h?mL -1 , AUC 0→∝ was 1325.90 and 2612.58ng?h?mL -1 respectively. Conclusion: This method can be used to study the pharmacokinetics of 6,7 dimethoxycoumarin derived from different traditional Chinese medicines containing this component.
