Effect of curcumin on primary cultured rat hepatocyte injurid by CCl_4
- VernacularTitle:姜黄素对四氯化碳损伤原代培养大鼠肝细胞的影响
- Author:
Yonggang LIU
;
Houchang CHEN
;
Jinjun ZHAO
;
Yiping JIANG
;
- Publication Type:Journal Article
- Keywords:
curcumin;
rat hepatocytes;
CCl 4
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of curcumin on carbon tetrachloridlde (CCl 4) intoxicated primary cultured rat hepatocytes. Methods:The rat hepatocytes were separated by perfusion with collagenase solution. Curcumin was added to the culture medium after incubating the rat hepatocyte for 36 hours. At the same time, CCl 4 was added to induce hepatocyte injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined, respectively. Results:Curcumin significantly improved cell viability, inhibited the decrease of SOD activity and increase of the ALT, LDH, MDA, NO levels. Conclusion: Curcumin protected effectively the primary cultured rat hepatocytes against injury induced by CCl 4.
