Determination of alliin in mice plasma by HPLC
- VernacularTitle:HPLC法测定小鼠血浆中蒜氨酸的浓度
- Author:
Junmin CHANG
;
Jian CHEN
;
Lijing ZHANG
;
- Publication Type:Journal Article
- Keywords:
HPLC;
alliin;
plasma concentration
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop HPLC method for determination of alliin concentration in mice plasma. Methods: The plasma samples were extracted with methanol. The analysis involved a ODS 1 column as stationary phase and distilled water as mobile phase. The flow rate was 0.5mL?min -1 , UV detection wavelength was at 220nm. 5 Fu was used as the internal standard. Results: The calibration curve was linear over a range from 14.8?g?mL -1 to 148.1?g?mL -1 with a correlation coefficient of 0.9992. Conclusion: The method is economic, simple, sensitive and accurate.
