Mitochondrial DNA instability and integration of mtDNA in the nuclei of gastric mucosa related to Helicobacter pylori infection
- VernacularTitle:胃黏膜细胞线粒体DNA不稳定及核内整合与幽门螺杆菌感染有关
- Author:
Xianlong LING
;
Dianchun FANG
;
Xiaodong ZHOU
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Carcinogenesis of gastric mucosa;
Helicobacter pylori;
Mitochondrial microsatellite instability;
Integration of mtDNA;
In situ hybridization
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Mitochondrial DNA (mtDNA) may be the target of oxygen free radicals related to Helicobacter pylori (H.pylori) infection, partly because of lack of protective histones and partly because of inefficient DNA repair systems. In this study we investigated the relationship between mitochondrial microsatellite instability (mtMSI) as well as integration of mtDNA in the nuclei of gastric mucosa and H.pylori infection. Methods H.pylori was detected using PCR and modified Giemsa staining. The mtMSI and the sequences of mtDNA in the nuclei were detected by PCR SSCP and in situ hybridization. Results The mtMSI was detected in 11 of 30 (36.7%) cases of gastric cancers, 2 of 15(13.3%) of intestinal metaplasia, 2 of 10 of dysplasia and 1 of 10 of chronic atrophic gastritis. The integration of mtDNA in the nuclei was detected in 20.0% (6/30) of gastric cancer, 1/10 of dysplasia, 6.7%(1/15)of intestinal metaplasia and 1/10 of chronic atrophic gastritis. The frequencies of mtMSI and integration of mtDNA in H.pylori positive group (12/39 and 8/39) were each significantly higher than those in H.pylori negative group (4/36 and 1/36, P 0.05), mtMSI and integration of mtDNA in the nuclei tended to occur in gastric mucosa with cagA positive H.pylori infection(10/25,6/25), but less often to occur in gastric mucosa with cagA negative H.pylori infection(2/14, 2/14). Conclusions The mtMSI and integration of mtDNA may be involved in the carcinogenesis of gastric mucosa and H.pylori infection might contribute to the mtMSI and integration of mtDNA.
