Quantitative determination of limonin in traditional Chinese medicine by LC-MS/MS
- VernacularTitle:中草药及制剂中吴茱萸内酯的LC-MS/MS测定方法
- Author:
Gangyi LIU
;
Guoquan WANG
;
Hui ZHANG
;
Chen YU
;
Shen JI
;
Dayuan ZHU
;
- Publication Type:Journal Article
- Keywords:
limonin;
fructus evodiae;
LC MS/MS
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a simple LC MS/MS method for the measurement of limonin in fructus evodiae, cortex phellodendri, cortex dictamni and its preparation. Methods: Separation was obtained by using a discovery C 18 column (150mm?2.1 mm), The mobile phase:A:water-0.1%HAc;B:acetonitrile-0.1%HAc;The elution program was gradient and the flow rate was 0.2mL?min -1 . The mass detector was set MS1(IS)=187.0 and MS2(Limonin)=471.0 by multiple reaction monitor(MRM) respectively.The sample was added psoralen(IS), then extracted with methanol chloroform(1∶3).Results: Limonin was linear from 25 to 1000ng?mL -1 ( r = 0.9996), The regression equation was C=3.124 As/Ai 1.935?10 -3 . The average recovery was 100.2%.Conclusion: This method is simple, quick, sensitive and exclusive.
