Determination of diosgenin in Rhizoma Paridis by HPLC and TLC
- VernacularTitle:高效液相色谱法和薄层色谱法测定重楼中薯蓣皂苷元的含量
- Author:
Huifen LI
;
Yuan PING
;
Gang CHEN
;
Junyan LI
;
Jun HE
;
- Publication Type:Journal Article
- Keywords:
Rhizoma Paridis;
diosgenin;
HPLC;
TLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To determine the contents of diosgenin in Rhizoma Paridis by HPLC and TLC. Methods ODS column was used in HPLC with mobile phase: acetonitrile water(90∶10), detection wavelength: 203 nm, column temperature: 35 ℃; silica gel plate was used in TLC with developer: cyclohexane ethyl acetate (4∶1), scan wavelength: 610 nm, reference wavelength: 420 nm. Results The linear ranges of HPLC and TLC were 1 2 - 7 2 ?g (r=0 999 8), 0 2 - 1 0 ?g (r=0 995 7). The average recovery and RSD were 102 2%, 3 39% (n=6) and 100 9%, 2 68% (n=6) respectively. Conclusion Both of two methods can be used for the quality control of diosgenin in Rhizoma Paridis.
