Detection of M2 in diagnosis of antimitochondrial antibody-negative patients with primary biliary cirrhosis using recombinant autoantigens from human sources
- VernacularTitle:人源M2抗原检测M2抗体诊断抗线粒体抗体阴性原发性胆汁性肝硬化
- Author:
Xiaohua JIANG
;
Renqian ZHONG
;
Feng AN
;
Yin HU
;
Xiantiao KONG
;
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis,biliary;
Autoantibodies;
Diagnosis
- From:
Chinese Journal of Rheumatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the biochemical and clinical features of antimitochondrial antibody (AMA) positive and negative primary biliary cirrhosis (PBC),and to compare the sensitivity of both the detection of M2 antibody using enzyme linked immunosobent assay (ELISA) with antigen expressed from human sources,and the detection of AMA using conventional indrect immunofluorecence for diagnosing PBC.Methods AMA and M2 antibodies were tested in 63 patients with diagnosed PBC.Results Serum AMA positive rate in PBC patients was 81%(51/63) compared with 100%(63/63) of M2 positive rate.Antinuclear and anti smooth muscle antibodies(ANA and ASMA) were more frequently positive in the AMA negative patients (83% vs 35%,and 42% vs 4%, P
