HPLC separation and determination of bufadienolide in Cinobufacini Injection
- VernacularTitle:华蟾素注射液中蟾毒内酯类成分含量检测
- Author:
Yonghua SU
;
Xueqiang HUANG
;
Dazhi ZHANG
;
Yani ZHANG
;
Juemin XIE
;
Changquan LING
;
- Publication Type:Journal Article
- Keywords:
cinobufacini;
bufalin;
resibufogenin;
cinobufagin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determinate the content of bufalin,resibufogenin and cinobufagin in Cinobufacini Injection. Methods: Liposoluble components in cinobufacini were extracted with ethyl acetate and determined by HPLC using a C 18 column, acetonitrile water(50∶50) as a mobile phase and UV detection wavelength at 299nm. Results: The method showed the good resolution, high sensitivity, satisfactory accuracy and specificity. Quantitatively analyze results showed that the concentration of bufalin, cinobufagin and resibufogenin in Cinobufacini Injection were 0.333 ?g?mL -1 , 0.159?g?mL -1 and 0.110?g?mL -1 , respectively. Conclusion: Bufalin in Cinobufacini Injection reached effective concentration, which was regarded as one of anti cancer components.
