Identification of Gegen qinlian preparation by three dimensinal HPLC
- VernacularTitle:葛根芩连制剂的三维高效液相谱鉴定
- Author:
Zhe LI
;
Hui LI
;
Fakui CHEN
;
Jianhui GAO
;
- Publication Type:Journal Article
- Keywords:
Gegen Qinlian Preparation;
puerarin;
baicalin;
berberine;
Three Dimensional HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the identification method of Gegen Qinlian Preparation(Radix Puerariae, Radix Scutellariae, Rhizoma Coptidis, and Radix Glycyrrhizae) by three dimensional HPLC. Methods: Puerain, baicalin and berberine were dissolved with 70% methanol as a reference solution. Gegen Qinlian preparation was analysed by three dimensional HPLC, methanol water (27∶73 containing 0.7‰ sodium lauryl sulfate and 0.7‰ triethylamine) as a mobile phase was used by linear gradient elution. Conclusion: The scientific and well defined identification of three dimensional HPLC can be showed by Gegen Qinlian Preparation research and this method is accurate and reliable.