Effect of melittin on proliferation of hepatocarcinoma cell line SMMC7721
- VernacularTitle:蜂毒素对肝癌细胞系SMMC7721增殖的影响
- Author:
Chen ZHANG
;
Changquan LING
;
Bai LI
;
Yonghai PENG
;
- Publication Type:Journal Article
- Keywords:
melittin;
hepatocarcinoma;
proliferation
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the effect of melittin on growth and proliferation of hepatocarcinoma cells. Methods Hepatocarcinoma cell line SMMC7721 was treated by melittin. The growth and proliferation was observed by cell counting and MTT assay; necrosis was estimated by Trypan blue staining. The expression of proliferating cell nuclear antigen (PCNA) and cycle phase were detected by flow cytometer. Results Melittin could significantly inhibit the growth of SMMC7721 and block up cells progress through G 2/M. The percentage of PCNA positive cell was decreased gradually when concentration of melittin was increased. Conclusion Melittin could inhibit the proliferative activity of hepatocarcinoma cell line SMMC7721 and reduce of PCNA positive cell expression and arrest cells in S phase.
