Identification by measuring internal transcribed spacer regions of rRNA gene in Radix Angelicae Sinensis
- VernacularTitle:rRNA基因间隔区碱基测序对当归进行鉴定的研究
- Author:
Keping JI
;
Yingdong LI
;
Xiling ZHANG
;
Xiaohong LI
;
- Publication Type:Journal Article
- Keywords:
Radix Angelicae Sinensis;
nested PCR;
to measure the sequence of DNA;
rRNA gene;
molecular identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To set up an identified standard on the level of molecule through measuring the internal transcribed spacer regions of the rRNA gene in Radix Angelicae Sinensis Methods To extract DNA from the seeds of Radix Angelicae Sinensis by conventional method, and use composed peculiar primer amplify with the internal transcribed spacer regions of the rRNA gene, to measure the base sequence of the amplified products Results It is proved by agar sugar gel electrophoresis that the PCR amplified products of the internal transcribed spacer regions of the rRNA gene exist The base sequence of the seeds of Radix Angelicae Sinensis internal transcribed spacer regions of the rRNA gene was measured Conclusion To measure the base sequence of internal transcribed spacer regions of the rRNA gene is another effective method to identify the vegetal traditional Chinese medicine on the molecular level
