Determination of isofraxidin in Xiaoerkaiwei Oral Liquid by HPLC
- VernacularTitle:高效液相色谱法测定小儿开胃口服液中异嗪皮啶含量
- Author:
Chao YU
;
Kang LING
;
Hongmei LAN
;
Yu WANG
;
Qiang YU
;
- Publication Type:Journal Article
- Keywords:
Isofraxidin;
RP HPLC, Radix Acanthopanasis senticosi
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determination of isofraxidin in Xiaoerkaiwei Oral Liquid (Radix Acanthopanacis Senticosi, Endothelium Corneum Gigeriae Galli, Semen Alpiniae Katsumadai, Semen Pharbitidis, etc.) by RP HPLC. Methods: The HPLC system consisted of Symmetry C 18 column(3.9m?150mm, 5?m), methanol water (0.1% formic acid) (30∶70) mixture as a mobile phase, detection wavelength at 342nm, 0.8mL?min -1 of flow rate and column temperature at 30℃.Results: The calibration curves of the isofraxidin were linear ( r =0.9993). The precision were perfect ( RSD =1.87). The samples were stable in 6 h. The average recovery of Xiaoerkaiwei Oral Liquid was 102.4% with RSD of 1.76%.Conclusion: This method is simple, quick and specific, suitable for the quality control.
