Determination of loureirin A and B extracted from Sanguis Draxonis in two extract processed by RP-HPLC
- VernacularTitle:不同工艺提取的龙血竭中龙血素A、B的含量的测定
- Author:
Yingqing HU
;
Qingyun ZHANG
;
Xiaoguo HU
;
Changhu DENG
;
- Publication Type:Journal Article
- Keywords:
Sanguis Draxonis;
loureirin A;
loureirin B;
HPLC;
heating free technology
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish HPLC method for the determination of loureirin A and loureirin B in Sanguis Draxonis Capsules.Methods: HPLC system included C 18 reverse phase column and acetonitrile 1% acetic acid (34∶66) as mobile phase, detection at 280nm and external standard method.Results:The standard curves of loureirin A was linear in the concentration range of 98～490ng, r =0.9996.The average recovery was 97.45%, RSD was 1.82%.The standard curves of loureirin B was linear in the concentration range of 43.2～259.2, r = 0.9993 .The average recovery was 96.92%, RSD was 1.57%.The contents of loureirin A and loureirin B in Sanguis Draxonis from heating free technology were higher than traditional technology.Conclusion: This heating free technology is a new technique that is a creation with higher extract rate,this method is worth populariging.
