Determination of troxerutinum and coumarin in Keyanzhong Tablets by HPCE
- VernacularTitle:高效毛细管电泳法测定克炎肿片中维脑路通和香豆素的含量
- Author:
Han QIU
;
Sujian CHEN
;
Feiyun YE
;
- Publication Type:Journal Article
- Keywords:
Keyanzhong tablets;
troxerutinum;
coumarin;
HPCE
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine troxerutinum and coumarin in Keyanzhong Tablets. Methods: A HPCE method is used. Ibuprofen is used as internal standard with the running buffer of 25 mmol?L -1 borax boric acid (pH9.2),containing 30 mmol?L -1 SDS and 10% acetonitrile. The running voltage is 20KV, at the detection wavelength of 214nm.Results: The linear ranges of Troxerutinum and Coumarin are 50 250?g?mL -1 ( r = 0.9991 ) and 25 125?g?mL -1 ( r =0.9993),respectively. The average recoveries are 98.4% and 98.8%, respectively. RSD are 1.6% and 2.0% ( n =5),respectively.Conclusion: The method is simple, accurate and sensible, and can be used for quality control of the preparation.
