Application of HPLC-MS to analysis of muscone
- VernacularTitle:HPLC-MS在麝香酮分析中的应用
- Author:
Haobing ZHANG
;
Yi TAO
;
Xiaokun HONG
;
Zhihua WANG
;
- Publication Type:Journal Article
- Keywords:
muscone;
standard addition;
HPLC MS
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To determine the content of muscone in preparation Methods Standard addition, DNP derivatization and HPLC with electrospray ionization tandem mass spectrometry (LC/ESI MS/MS) were utilized Results Identification of muscone hydrazone in the sample was based on the unique mass spectra of the standard Under an optimum condition peak corresponding to + ion and characteristic fragments for muscone hydrazone were observed ESI and APCI full scan m/z 419 MS/MS had the same relative molecular mass and fragmentation pattern The recovery was 102 5% with RSD ≤ 2 76% (n=5) Conclusion The qualitative and quantitative determination on muscone could be fulfilled simultaneously
