Tissue culture of stem segment and plantlet regeneration of Dendrobium nobile
- VernacularTitle:金钗石斛的茎段组织培养与植株再生
- Author:
Peiyuan GAO
;
Jianmiao CHEN
;
Quan GAN
;
- Publication Type:Journal Article
- Keywords:
Dendrobium nobile Lindl.;
tissue culture;
plantlet regeneration
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To speed up the artificial propagation of the costly medicinal plant Dendrobium nobile Lindl. Methods The tender stems with lateral buds were tried as the explants and cultured on various culture media with different portions of hormon. Results The best medium for bud induction was the MS basic medium with addition of 6 BA (0.5 mg/L), NAA (0.2 mg/L), while MS with the addition of 6 BA (3.0 mg/L) NAA (0.5 mg/L), was suitable for propagation, MS with addition of IBA (0.3 mg/L), NAA (0.1 mg/L) was better for root growing, the striking growth rate was up to 95%. The young sprout was cultured fine on the base material mixed with coco crumbs, volcanic rocks and brick fragments (1∶1∶ 1) and covered by dried pteridophyte. Conclusion To provide the effective ways to reserve the resources of D. nobile and last its utlization.
