Determination of forsythin in Shuanghuanglian Oral Liquid by RP-HPLC
- VernacularTitle:RP-HPLC测定双黄连口服液中连翘苷含量
- Author:
Yong CHEN
;
Binghui ZHU
;
Jihui FANG
;
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian Oral Liquid;
RP SPE;
RP HPLC;
forsythin;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determination of forsythin in Shuanghuanglian Oral (Flos Lonicerae, Radix Scutellariae, Fructus Forsythiae, etc.) Methods: After processing by RP SPE (reverse phase solid phase extraction), the sample solution was measured by RP HPLC. The chromatographic conditions were: Prodigy ODS (3) (150?4.6mm,5?m) column as analytic column; MeOH H 2O HAc(40∶60∶1,V/V) as mobile phase; detection wavelength at 227nm; column temperature at 35?C ; flow rate at 1.0mL?min -1 . Results: The linear range of forsythin was 0.1～2.0?g, r =0.9997. The average recovery was 102.2%, RSD =0.61%( n =6). Conclusion: The method is simple, accurate, reproducible and can be used to enhance the quality control of this drug.
