Improved method of extracting RNA from Anoectochilus roxburghii
- VernacularTitle:金线莲RNA提取方法的改进
- Author:
Mingjuan TANG
;
Shunxing GUO
;
- Publication Type:Journal Article
- Keywords:
Anoectochilus roxburghii (Wall.) Ldl.;
phenolic compounds;
polysaccharides;
RNA
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Object Effectively extracting RNA from Anoectochilus roxburghii (Wall.) Ldl. lays a foundation to research the mechanism of endomycorrhiza promoting its growth. Methods RNA was prepared by CTAB, electrophoresis on 1% agroses gel and photo are taken with MutiImage TM Light Cabinet. Results The ratios of RNA A 260 /A 230 and A 260 /A 280 were 2.054 and 1.818, respectively. And the yield was 154.35 ?g/g fresh. The bands were clear on agrose gel and integrity of RNA was good. Conclusion RNA with high quality and high quantity is isolated by the method of CTAB for medicinal plants with high concentrations of polysaccharides and phenolics.
