FACTORS ASSOCIATED WITH THE EXPRESSION OF HUMAN ?-SYNUCLEIN GENE IN PROKARYOTIC CELLS
- VernacularTitle:影响人?-突触核蛋白基因在原核细胞中表达因素的研究
- Author:
Shuting LI
;
Biao CHEN
;
- Publication Type:Journal Article
- Keywords:
synuclein;
Induction;
DH5?/E.coli;
Expression
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study factors that may increase the expression of human ? synuclein gene in prokaryotic cells. Methods proEXNACP vector was transferred into competence cells. Factors such as IPTG levels, initial concentration of cells, type and pH of media, time and temperature of induction were studied in relation to the production of ? synuclein. Results IPTG concentration and pH change does not have a significance impact on ? synuclein production. But the time and temperature of induction, media and initial concentration of cells significantly affected the expression.Conclusion The best condition for the production of ? synuclein includes factors such as LB media with an initial concentration of cells of 0 5\|0 8 A at 37℃ culture with oxygen of 180?rpm/min for 1 2?hours.
