Determination of ferulic acid and paeoniflorin in Huoxue Mixture by HPLC
- VernacularTitle:活血合剂中阿魏酸和芍药苷的含量测定
- Author:
Qianzhou LU
;
Xinhong WANG
;
Rui AN
;
Jianzhong ZHANG
;
- Publication Type:Journal Article
- Keywords:
Huoxue Mixture;
RP HPLC;
ferulic acid;
paeocniflorin
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the determination of the contents of ferulic acid and paecniflorin in Huoxue Mixture Radix Salviae Miltiorrmizae, Radix Angelicae sinensis, Radix Paeoniae Rubra cortex Moutan, Radix Astragali and caulis Spatholobi simultaneously by HPLC.Methods:The determination was carried out with ZORBAX *ODS column. Chromatographic condition of ferulic acid: the mobile phase consisted of methanol 1%HAc (42∶58) and UV detection wavelength was at 313nm. Chromatographic condition of paecniflorin: the mobile phase consisted of methanol water (25∶75) and UV detection wavelength was 230nm.Results:There was a good linear relationship between the absorption peak area and the concentration in the rang of 0.125～1.0?g for ferulic acid and 0.25～2.0?g for paecniflorin, respectively.Conclusion:The method is simple, acurate, reproducible and can be applied for content determination of Huoxue Mixture.
