Establishment of method for the analysis of Yun Zhi Polysaccharopeptide(PSP) by HPCE
- VernacularTitle:云芝糖肽的HPCE分析方法的建立
- Author:
Ping YUAN
;
Xiaokun HONG
;
- Publication Type:Journal Article
- Keywords:
Yun Zhi Polysaccharopeptide(PSP);
HPCE(Capillary Zone Electrophoresis);
DAD detection;
characteristic electropherograms
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the analytical method for the characteristic electropherograms of PSP by High Performance Capillary Electrophoresis(Capillary Zone Electrophoresis). Method: Types, concentration and pH of electrolyte, voltage, temperature, length and size of column were investigated for the analysis of PSP and BSA which is as standard using capillary zone electrophoresis. Results: The optimum condition is that 10mmol?L -1 boric acid buffer, pH9.18, a column 50?m?57cm(HP, effective length of 48.5cm), 30KV, temperature 25 ?C ,DAD detection. The analysis is ended within 7min. Conclusion: The method is simple, rapid and reliable. The characteristic electropherograms of PSP can be established.
