Determination of ester-alkaloids in Shiwuweirupeng Capsules by RP-HPLC
- VernacularTitle:RP-HPLC测定十五味乳鹏胶囊中酯型生物碱的含量
- Author:
Jinhai YI
;
Yan CHEN
;
Yuhong LIU
;
Shixiang HOU
;
- Publication Type:Journal Article
- Keywords:
aconitium flavum;
ester alkaloids;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the determination method of ester alkaloids in Shiwuweirupeng Capsules. Methods:The chromatographic method was carried out on Aichrom TM C 18 column using CH 3OH 0.05 mol?L -1 KH 2PO 4 CH 3COOH-(CH 3) 2CHOH(67∶173∶4∶4) as a mobile phase. The detection wavelength was set at 230nm. The flow rate was 1mL?min -1 . The column temperature was 35?C. Results: The benzoic acid calibration curves were linear at the ranges of 0.0152～0.076?g( r =0.9998). The average recoveries of aconitine was 93.3% with RSD =1.9%.Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Shiwuweirupeng Capsules.
