Determination of flavonoids in tender stem and leaf of Ampelosis prossedentata by differential spectrophotometry
- VernacularTitle:差示分光光度法测定广西瑶族藤茶中黄酮类成分的含量
- Author:
Jieping QIN
;
Shandan LIANG
;
Cuiwei HE
;
- Publication Type:Journal Article
- Keywords:
Ampelosis grossedentata (Hand. Mazz.) W. T. Wang;
flavonoids;
dihydromyricetin;
differential spectrophotometry
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a method for the determination of flavonoids from the tender stem and leaf of Ampelosis grossedentata (Hand. Mazz.) W. T. Wang, and for its quality control. Methods Differential spectrophotometry was used at the wavelength of (318?1) nm, while taking dihydromyricetin as index. Results The regression equation, within the range of the control concentration in 4 576～22 88 ?g/mL, was A=29 56 C+0 005 5, with a good linearity (r=0 999 8) between the absorbance and concentration. The average recovery rate was 100 8% and RSD was 1 9% (n=5). Conclusion The method is simple, reliable and easy to operate and suitable for the quality control of Chinese medicinal materials with a better reproducibility.
