Isolation of total DNA from Plant Chinese medicinal materials
- VernacularTitle:植物药材总DNA提取
- Author:
Xiaobo LI
;
Bo FENG
;
Zhaohui ZHANG
;
Zhengtao WANG
;
Luoshan XU
;
- Publication Type:Journal Article
- Keywords:
DNA extract;
plant Chinese medicinal materials;
Cimicifugae foelida L.
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata (Slms) Kostern., and Akebia quinata Decne. etc This method is preferable in removing pigments, polysaccharides, substance disturbing PCR Conclusion In this process, common reagents are used, lower cost is of advantage to popularize This study provids the applying foundations for the DNA identification of plant Chinese medicinal materials
