Effect of Aromatic Herbs for Resuscitation on NO Content and Expression of NOS in Brain Tissue of Rats with Cerebral Ischemia and Reperfusion Injury
- VernacularTitle:芳香开窍法对全脑缺血再灌注大鼠脑组织NO含量及NO合酶表达的影响
- Author:
Ci'An ZHANG
;
Yamin LIU
;
Qiuying XU
;
Shengquan PENG
;
- Publication Type:Journal Article
- Keywords:
AROMA RESUSCITATION;
CEREBRAL ISCHEMIA /TCD therapy;
NITRIC OXIDE;
NITRIC OXIDE SYNTHASE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the effect of aromatic herbs for resuscitation such as Moschus and Borneol on nitric oxide (NO) content and expression of endothelial NO synthetase (eNOS) and inducible NO synthetase (iNOS) in brain tissue of rats with cerebral ischemia and reperfusion injury. [Methods] Forty rats were allocated to 4 groups: mimic operation group (Group A), model group (Group B), herbs treatment group (Group C) and nimodipine treatment group (Group D). Rat model of cerebral ischemia and reperfusion injury was established by Pulsinelli 4VO method. NO content in brain tissue was determined by nitrate reductase method. Expression of eNOS and iNOS in micrangium and iNOS in neurons was measured by semi-quantitative analysis. [Results] NO content was decreased and approached normal level in Group C and Group D (P 0.05 as compared with Group A). Aromatic herbs with action of inducing resuscitation increased the expression of eNOS in micrangium and decreased the expression of iNOS in micrangium and neurons, the differences being significant as compared with those in Group B ( P
