Study on extraction process of chlorogenic acid in Flos Lonicerae by enzymic treatment
- VernacularTitle:金银花绿原酸酶法提取新工艺研究
- Author:
Jaja LIU
;
Guoling ZHAO
;
Xiaohua ZHANG
;
Hui WANG
;
- Publication Type:Journal Article
- Keywords:
Flos lonicerae;
Chlorogenic acid;
enzymic treatment;
extraction process
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extraction process of chlorogenic acid in flos lonicerae.Methods: The flos lonicerae was treated by means of cellulase and/or pectolase before extracted by ethanol reflux, studying the enzyme dosage, treatment time, treatment temperature how to influence the percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction.Results: The percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction could be obviously increased by the cellulase treatment, 8.15g chlorogenic acid could be extracted from 100g flos lonicerae. The optimum temperature of treatment was 40℃～50℃; The percentage of extraction was higher by the combined treatment of cellulase and pectilase than by cellulase only, but the amount of chlogenic acid was not obviously different between these two methods.Conclusion: The amount of chlorogenic acid in extrction could be increased by enzymic treatment.
