Determination of cinnamaldehyde and cinnamic acid in Ramulus Cinnamomi and Guizhifuling Capsules by RP-HPLC
- VernacularTitle:RP-HPLC法测定桂枝及桂枝茯苓胶囊中桂皮醛和桂皮酸含量
- Author:
Song YANG
;
Baofeng JIN
;
Zhiguo ZHANG
;
Kaishun BI
;
- Publication Type:Journal Article
- Keywords:
Ramulus cinnamomi;
Guizhifuling capsules;
cinnamaldehyde;
cinnamic acid;
RP HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine cinnamaldehyde and cinnamic acid in Ramulus Cinnamomi and Guizhifuling Capsules.Methods: RP HPLC method was used. The samples were separated on a Zobax ODS column with the mobile phase of acetonitrile:0.1% phosphoric acid (34∶76) and the detection wavelength was set at 285nm.Results: The calibration curves were linear in the range of 3.34～53.4?g?mL -1 in cinnamaldehyde ( r= 0.9996) and 0.804～12.9?g?mL -1 in cinnamic acid ( r =0.9999),respectively. The average recoveries of cinnamaldehyde in Ramulus cinnamomi and Guizhifuling capsules were 98.6% ( RSD =1.35% n=6 ) and 97.4% ( RSD =1.62% n =6), respectively and of cinnamic acid were 99.2% ( RSD =0.97% n=6 )and 100.0% (RSD =0.73%, n=6 ),respectively.Conclusion: The method is quick, acurate and suitable for the determination of Ramulus Cinnamomi and its compound Chinese medicinal preparations.
