Determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem from different habitats by HPLC
- VernacularTitle:HPLC测定不同产地功劳木中盐酸巴马亭及盐酸小檗碱的含量
- Author:
Jie HUANG
;
Tong SANG
;
Hongping QIN
;
- Publication Type:Journal Article
- Keywords:
Chinese Mahonia Stem;
palmatine hydrochloride;
berberine hydrochloride;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To develop a method for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem from different habitats Methods HPLC method was set up, using Intersil ODS 3 C 18 column, the mobile phase was acetontrile water sodium laurylsulfonate (470∶ 530∶1 g), the UV detection wavelength was 265 nm, with a flow rate of 1 0 mL/min at 40 ℃ Results A good linearity was obtained in the range of 4 368 52 416 ?g/mL(r=0 999 9) for palmatine hydrochloride and 4 532 54 384 ?g/mL (r=0 999 9) for berberine hydrochloride The average recovery of palmatine hydrochloride and berberine hydrochloride was 98 97% and 98 98%, respectively Conclusion The method is simple, rapid and with better reproducibility for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem
