Determination of rhien, emodin, and chrysophanol in Huangdanyinchen Granules by HPLC
- VernacularTitle:HPLC同时测定黄疸茵陈颗粒中大黄素、大黄酸、大黄酚的含量
- Author:
Xiaodong HUA
;
Zhihua WANG
;
Jumei WANG
;
Qin XIE
;
- Publication Type:Journal Article
- Keywords:
Huangdanyinchen Granules;
rhien;
emodin;
chrysophanol;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. Methods: HPLC was used to determine the contents of emodin, rhien and chrysophanol in Huangdanyinchen Granules. The separation was performed on YWG ODS column with methol perchloric acid (1%) (8∶2) as a mobile phase and the wavelength of UV detector was 280nm.Results: The separating degree and the linearity of this method was well with the average recovery of rhien: 97.43%, RSD= 1.90%; Emodin:98.45%,RSD=1.62%;Chrysophanol: 98.87%, RSD=1.50%, respectively.Conclusions:The method is simple and rapid and gives satisfactory results. It can be suitable for quality controll of Huangdanyinchen Granules.
