Screening for antiviral fractions from ethanol extract of Euphorbia Kansui Liou
- VernacularTitle:甘遂醇提物抗流感病毒FM_1有效部位的筛选
- Author:
Weifa ZHENG
;
Caifa CHEN
;
Aihua ZHU
;
Mengqiu LI
;
- Publication Type:Journal Article
- Keywords:
Euphorbia kansui;
initial fractions of ethanol extract;
antiviral fractions;
lymphocyte proliferation
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To elucidate antiviral fractions from ethanol extract of Euphorbia kansui .Methods: Initial separation of ethanol extract from Euphorbia kansui was performed by column chromatography. The test mice were infected with flu virus mouse pneumonia adaptive strain (FM 1) and then treated with separated fractions intragastrally for determining lung index inhibition rate. The T lymphocyte proliferation enhancement was observed by the dosimetry of 3 H TdR infiltrated into DNA of the cell.Result: Initial separation of the extract afforded to 15 fractions. Fractions 8～15 with the elution volume from 29500 to 95000mL were highly effective to the therapy of the mice pneumonia. The enhancement to the lymphocyte proliferation of the 8 fractions under low concentration were 2～3 times higher than that of the control.Conclusion: Fractions 5 and 8～15 exhibited strong in vivo antiviral activity to the test virus, which might be realized by stimulating lymphocyte proliferation, enhancing the capacity of killing the cells infected by the virus.
