Studies on conditions of tissue culture and accumulation of alkaloids at different period in Fritillaria mellea
- VernacularTitle:浓蜜贝母的组织培养条件及不同时期生物碱积累的研究
- Author:
Yufeng LI
;
Fang YAN
;
Lin TANG
;
Ying XU
;
Fang CHEN
;
- Publication Type:Journal Article
- Keywords:
Fritillaria mellea S. Y. Tang et S. C. Yueh;
tissue culture;
callus;
induction;
alkaloid
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To compare the contents of alkaloids of callus at different period with tissue culture of fresh bulbs of Fritillaria mellea S. Y. Tang et S. C. Yueh.Methods To study the best condition of induction frequency of callus about some phytohormones, temperature and light, and then to determine the contents of alkaloids of callus in F. mellea. Results The best condition of induction frequency of callus above was NAA 1 mg/L+ 6BA 3 mg/L; temperature at (22?1) ℃ and scattered light; the contents of alkaloids were kept highest in about 20th day ofter the callus appeared.Conclusion The contents of alkaloids of callus are kept higher level than fresh bulbs of F. mellea.
